SCIENCE
CerFlux science advances human-relevant New Approach Methodologies (NAMs) that combine biomimetic engineering, microphysiological systems, and high-throughput ex vivo and in silico (computational and AI/ML) tumor models to better understand how individual tumors respond to therapy before treatment begins.
This page highlights peer-reviewed publications, high-impact conference abstracts, issued patents, and funded research that underpin platforms such as POET, BEST, SMART, and Lab-on-a-Brane technologies. Together, these efforts reflect a commitment to building human-relevant technologies that support more informed, patient-specific decision-making across translational cancer research and development.
Measuring surface and interfacial tension in situ in Microdripping Mode for electrohydrodynamic applications
Budhwani, KI; Pekmezi, GM; Selim, MM.
Publication | Micromachines | 2020 | DOI: 10.3390/mi11070687

Surface tension is what lets small insects seem to “walk on water”—and in electrohydrodynamic atomization (EHDA), the same physics strongly shapes droplet formation. In this paper, we present an in situ method to estimate surface and interfacial tension (S/IFT) directly within EHDA workflows (eg, electrospinning/electrospraying), where conventional techniques (pendant drop, ring/plate) are often impractical to apply. Our approach uses signal-processing algorithms to extract droplet frequency/periodicity in EHDA microdripping mode and maps those measurements to computational fluid dynamics (CFD) solutions to infer S/IFT during operation. We validate the method against published ranges across multiple reference interfaces and show it captures expected trends such as reduced surface tension with increasing surfactant concentration, while explaining offsets relative to traditional methods. Overall, this provides a practical measurement workflow for tuning EHDA-based processes relevant to microphysiological systems and drug-delivery manufacturing.
Bridging the Gap in Training and Clinical Practice in Sub-Saharan Africa
Saleh, MN; Naik, G; Mwirigi, A; Shaikh,AJ; Sayani, S; Ghesani, M; Asaria, S; Sohani, AR; Sayed, S; Moloo, Z; Budhwani, KI; Talib, Z.
Publication | Current Breast Cancer Reports | 2019 | DOI: 10.1007/s12609-019-00322-6

In this paper, we describe a growing gap in cancer care between low- and middle-income countries (LMICs) and high-income countries (HICs): LMIC settings often lack basic preventive and diagnostic services for early cancer, while HICs have greater access to novel diagnostic and therapeutic modalities. We argue that narrowing this disparity will require innovative technology, knowledge sharing, and sustained public–private partnerships that can bridge geographic and resource constraints. A key emphasis is the value of onsite and online training programs to strengthen regional capacity and translate modern oncology practices into routine care. We also share case studies illustrating practical ways these collaboration and training models can help close the gap.
Lab-on-a-Brane: Biomimetic interface device and methods of using the same
Budhwani, KI.
Issued Patent | USPTO | US10114010B1 | Filed 2016 | Approved 2018

This issued patent describes a biomimetic apparatus configured to simulate physiological conditions by providing configurable barrier and transport interfaces within a closed-loop fluid-flow assembly. The modular cassette-based architecture enables controlled flow and transport across an engineered interface to better approximate in vivo dynamics where barrier function matters. Designed to be reconfigurable and scalable, the platform supports human-relevant experimental workflows such as transport studies and compound/therapeutic evaluation in systems that depend on realistic interface behavior.
Novel Biomimetic Microphysiological Systems for Tissue Regeneration and Disease Modeling
Budhwani, KI; Oliver, PG; Buchsbaum, DJ; Thomas, V.
Book Chapter | Advances in Experimental Medicine and Biology | 2018 | DOI: 10.1007/978-981-13-0947-2_6

In this publication, we introduce a new class of biomimetic microphysiological systems that addresses a fundamental shortcoming of conventional lab-on-chip platforms: the absence of tissue-relevant porous barriers that govern real biological transport and interface behavior. By integrating electrospun nanofibrous porous scaffolds directly into microfluidic systems, we developed a lab-on-a-brane (LOB) architecture that more faithfully reproduces key structural and functional features of native extracellular matrix. This platform enables more realistic investigation of molecular transport, air–liquid interfaces, and tumor progression, establishing a scalable and versatile foundation for advanced disease modeling and translational research. The work underscores the CerFlux approach to building physiology-first systems designed to push beyond incremental improvements and redefine what predictive models can achieve.
